Gateway Capital Achieves First Close of $25M Fund II, Targets Midwest AI and Supply Chain Startups

Gateway Capital’s Strategic Expansion: First Close of $25M Fund II

Gateway Capital Partners, a Milwaukee-based venture firm founded by Dana Guthrie, has achieved the first close of its ambitious $25 million Fund II. This milestone enables the firm to commence investment operations, marking a significant step in its mission to support early-stage startups.

Established in 2020, Gateway Capital initially launched with a $13 million Fund I, focusing on nurturing nascent companies within the Midwest. The success of this inaugural fund has paved the way for the current fundraising endeavor. While the exact amount secured in this first close remains undisclosed, the firm is poised to make substantial investments in the near future.

Dana Guthrie, the visionary behind Gateway Capital, began the fundraising journey for Fund II in mid-2025. The firm plans to issue average check sizes ranging from $500,000 to $600,000, demonstrating a commitment to providing meaningful support to emerging enterprises. Although Fund II maintains an industry-agnostic stance, there is a deliberate emphasis on sectors within the Midwest that are primed for innovation and disruption. Key areas of interest include supply chain logistics and manufacturing artificial intelligence (AI), reflecting the region’s industrial strengths and potential for technological advancement.

Guthrie’s strategic vision encompasses backing at least 20 companies through this fund, aiming to catalyze growth and innovation within the Midwest’s entrepreneurial ecosystem. By targeting industries ripe for transformation, Gateway Capital seeks to foster a new wave of startups that can redefine traditional sectors and contribute to the region’s economic vitality.

The firm’s approach aligns with a broader trend of venture capital focusing on underrepresented regions and industries, recognizing the untapped potential and opportunities for substantial returns. Gateway Capital’s commitment to the Midwest underscores a belief in the region’s capacity to produce groundbreaking companies that can compete on a national and global scale.

As Gateway Capital embarks on this new chapter with Fund II, the firm stands as a beacon for aspiring entrepreneurs in the Midwest, offering not just capital but also strategic guidance and support. The emphasis on sectors like supply chain logistics and manufacturing AI indicates a forward-thinking approach, anticipating the future needs and challenges of these industries.

In conclusion, the first close of Gateway Capital’s $25 million Fund II marks a pivotal moment for the firm and the broader Midwest startup landscape. With a clear focus on industries poised for disruption and a commitment to substantial investment in early-stage companies, Gateway Capital is set to play a crucial role in shaping the future of innovation in the region.