AI Startups in the U.S. Secure Over $100 Million in 2025: A Year of Unprecedented Growth
The artificial intelligence (AI) sector in the United States experienced remarkable growth in 2025, with 55 startups each securing funding rounds of $100 million or more. This surge underscores the escalating investor confidence and the rapid advancement of AI technologies across various industries.
December Highlights:
– Mythic: Based in Austin, Texas, Mythic specializes in power-efficient computing solutions for AI applications. On December 17, the company announced a $125 million venture round led by DCVC, with participation from SoftBank, NEA, and Linse Capital.
– Chai Discovery: This biotech-focused AI firm secured a $130 million Series B round on December 15, valuing the company at $1.2 billion. The round was co-led by Oak HC/FT and General Catalyst.
– Fal: A generative media platform, Fal completed its third funding round of the year on December 9, raising $140 million in a Series D round led by Sequoia, bringing its valuation to over $4.5 billion.
– Unconventional AI: On December 8, this innovative startup announced a substantial $475 million seed round led by Lightspeed Venture Partners and Andreessen Horowitz, valuing the one-year-old company at nearly $4.5 billion.
– 7AI: Specializing in cybersecurity AI agents, Boston-based 7AI raised a $130 million Series A round on December 4, led by Index Ventures, with contributions from Greylock, Spark Capital, and CRV.
November Highlights:
– Genspark: An all-in-one AI workspace platform, Genspark announced a $275 million Series B round on November 20, valuing the company at $1.25 billion. Investors included Emergence Capital Partners, SBI Investment, and LG Technology Ventures.
– Luma AI: Focused on AI models for photo and video creation, Luma AI raised $900 million in a Series C round led by Humain, with participation from Andreessen Horowitz, AMD Ventures, and Amplify Partners, valuing the startup at $4 billion.
– Anysphere: The creator of the viral coding platform Cursor, Anysphere secured $2.3 billion in funding on November 13, bringing its valuation to $29.3 billion. This marks the company’s second funding round in 2025.
– Parallel: Specializing in web infrastructure for AI agents, Parallel announced a $100 million Series A round on November 12, co-led by Index Ventures and Kleiner Perkins.
– Hippocratic AI: A healthcare AI agent startup, Hippocratic AI raised a $120 million Series B round on November 10, led by General Catalyst, with participation from Andreessen Horowitz and GV.
October Highlights:
– Modal: An AI cloud computing platform founded in 2021, Modal raised an $87 million Series B round, achieving a valuation of $1.1 billion. Investors include Lux Capital and Redpoint Ventures.
– Fireworks AI: This AI infrastructure platform for open-source models secured a $250 million Series C round, valuing the company at $4 billion. Benchmark Capital and Index Ventures are among its investors.
– Substrate: A semiconductor fabrication company founded in 2022, Substrate raised a $100 million seed round, reaching a valuation of $1 billion. Valor Equity Partners and Founders Fund participated in the funding.
– LangChain: An agent engineering platform established in 2022, LangChain secured a $125 million Series B round, valuing the company at $1.3 billion. Sequoia and Benchmark Capital are among its investors.
– New Limit: A precision medicine company founded in 2021, New Limit raised a $45 million round, achieving a valuation of $1.6 billion. Kleiner Perkins is among its investors.
September Highlights:
– Tempo: A blockchain payments company founded in 2025, Tempo raised a $500 million Series A round, reaching a valuation of $5 billion. Thrive Capital is among its investors.
– Lila: A life scientific discovery company founded in 2023, Lila secured a $350 million Series A round, valuing the company at $1.3 billion. Braidwell and Collective Global are among its investors.
– Stoke: A sustainable rocket company founded in 2019, Stoke raised a $610 million Series D round, achieving a valuation of $2 billion. MaC Venture Capital and the US Innovation Technology Fund are among its investors.
– Apex: A company utilizing off-the-shelf components for building satellites, Apex raised a $200 million Series D round, reaching a valuation of $1 billion. Andreessen Horowitz and 8VC are among its investors.
August Highlights:
– EliseAI: A healthcare and housing automation platform, EliseAI raised a $250 million Series E round on August 20, valuing the startup at $2.2 billion. The round was led by Andreessen Horowitz.
– Decart: An AI research lab, Decart raised a $100 million round on August 7, achieving a valuation of $3.1 billion. Investors include Sequoia Capital, Benchmark, and Zeev Ventures.
July Highlights:
– Fal: A generative media platform, Fal raised a $125 million Series C round on July 31, led by Meritech Capital Partners, valuing the company at $1.5 billion.
– Ambience Healthcare: Building an AI healthcare operating system, Ambience Healthcare raised a $243 million Series C round, led by Oak HC/FT and Andreessen Horowitz.
– Reka AI: An AI research lab, Reka AI raised $110 million in a Series B round on July 22, valuing the company at $1 billion.
– Thinking Machines Lab: Confirmed a $2 billion seed round on July 15, led by Andreessen Horowitz, valuing the company at $12 billion.
– OpenEvidence: Building an AI-powered search tool for clinicians, OpenEvidence raised $210 million in a Series B round on July 15, valuing the company at $3.5 billion.
– Harmonic: Developing a mathematical reasoning engine, Harmonic raised a $100 million Series B round on July 10, valuing the company at $875 million.
June Highlights:
– Glean: An enterprise search startup, Glean announced a $150 million Series F round on June 10, led by Wellington Management, with participation from Sequoia, Lightspeed Venture Partners, and Kleiner Perkins.
February Highlights:
– Together AI: Creating open-source generative AI and AI model development infrastructure, Together AI raised a $305 million Series B round on February 20, valuing the company at $3.3 billion.
– Lambda: An AI infrastructure company, Lambda raised a $480 million Series D round on February 19, valuing the startup at nearly $2.5 billion.
– Abridge: An AI platform transcribing patient-clinician conversations, Abridge was valued at $2.75 billion in a $250 million Series D round announced on February 17.
– Eudia: An AI legal tech company, Eudia raised $105 million in a Series A round on February 13, led by General Catalyst.
– EnCharge AI: An AI hardware startup, EnCharge AI raised a $100 million Series B round on February 13, led by Tiger Global.
– Harvey: An AI legal tech company, Harvey raised a $300 million Series D round on February 12, valuing the company at $3 billion.
January Highlights:
– ElevenLabs: A synthetic voice startup, ElevenLabs raised a $180 million Series C round on January 30, valuing the company at more than $3 billion.
– Hippocratic AI: Developing large language models for the healthcare industry, Hippocratic AI announced a $141 million Series B round on January 9, valuing the company at more than $1.6 billion.
The substantial investments in these AI startups reflect the growing importance and integration of artificial intelligence across various sectors, from healthcare and legal tech to media and infrastructure. As AI continues to evolve, these companies are poised to drive innovation and reshape industries in the coming years.
